The late Arleen Sorkin may be the definitive Harley Quinn, originating the role in Batman: The Animated Series after her friend Paul Dini wrote it just for her. Harley has evolved far beyond those roots, both in the comics and in subsequent adaptations where actors like Margot Robbie and Kaley Cuoco put their own spin on the character. But they never would have had their chance without Sorkin leading the way.
Source:https://www.cbr.com/arleen-sorkin-best-harley-quinn-performances/