Spider-Man: Across the Spider-Verse featured the animated Gwen Stacy/Spider-Woman even more than its predecessor, namely by showcasing the strained relationship between her and her father. Strangely enough, however, this is the only member of her family that's shown in the movie. This odd decision is one shared by another cinematic Spider-Man universe.
Source:https://www.cbr.com/spider-man-sequels-missed-gwen-stacy-development/