Spider-Man has one of the best comic supporting casts. His friends and loved ones, like Mary Jane Watson and his Aunt May, play significant roles in his life. The Web-Slinger also has one of the best rogue galleries, full of deadly and iconic villains such as the Green Goblin and Doctor Octopus. What's striking is how many of Spidey's villains have emerged from his personal circle of friends. A recurring trope in Spider-Man comics is the mystery villain, whose identity is often revealed to be someone close to Peter Parker. When this occurs, Spidey feels a deep sense of betrayal, because nothing hurts him more than those nearest and dearest to him seeking his demise.
Source：https://www.cbr.com/spiderman-biggest-traitors-marvel/
